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SARS-CoV-2 Neutralizing Antibody Elisa Kit

Neutralizing ELISA For Antibody Discovery,neutralizing antibody Supplier
SARS-CoV-2 (COVID-19) Neutralizing Antibody Detection Kit(ELISA ) is an indirectenzyme-linked immunoassay (ELISA) for quantitative determination of coVID-19Ncutralizing Antibody in plasma or serum. This kit is used for assisted diagnosis.
  • 96 T / kit

  • Hunan Runmei Gene

  • L/C, T/T, D/P, Western Union, Paypal

  • CE, ISO 13485

  • Neutralizing Ab

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Product Description

SARS-CoV-2 (COVID-19) Neutralizing Antibody Detection Kit(ELISA ) is an indirectenzyme-linked immunoassay (ELISA) for quantitative determination of COVID-19 Ncutralizing Antibody in plasma or serum. This kit is used for assisted diagnosis.


Product Features

  1. High specificity

  2. High sensitivity

  3. High stability



Priciple of the assay 


SARS-CoV-2 (COVID-19)Neutralizing Antibody Detection Kit(ELISA ) is for rapid Nab(neutralizing antibody) screening, which can mimic the virus neutralization process. The kitcontains two key components: the Horseradish peroxidase(HRP) conjugated recombinant SARS-CoV-2 RBD fragment (HRP-RBD) and the human ACE2 receptor protein (hACE2). The protein-protein interaction between HRP-RBD and hACE2 can be blocked by neutralizing antibodiesagainst SARS-CoV-2 RBD.

First, the samples and controls are pre-incubated with the HRP-RBD to allow the binding of thecirculating neutralization antibodies to HRP-RBD. The mixture is then added to the capture platewhich is pre-coated with the hACE2 protein.The unbound HRP-RBD as well as any HRP-RBDbound to non-neutralizing antibody will be captured on the plate,while the circulatingncutralization antibodies_HRP-RBD complexes remain in the supernatant and get removedduring washing.After washing steps,TMB solution is added.By adding Stop Solution,thereaction is quenched. This final solution can be read at 450 nm in a microtiter plate reader. Theabsorbance of the sample is inversely dependent on the titer of the anti-SARS-CoV-2 neutralizingantibodies.


Sample requirements


Applicable sample types: Serum or plasma.

Handling and storage of samples:

·Follow procedures within your laboratory to avoid cross contamination of patient specimens.·For human/animal plasma, treat blood with anticoagulant such as citrate,EDTA or heparin.- Sample inactivate under 56°C for 30min can be used.

· Samples may be stored at room temperature (15-30°C) for no longer than 8 hours. If the test willnot be completed within 8 hours, refrigerate the serum samples at 2-8°C for no longer than 8 days.·Run assay immediately, otherwise store samples below -18°C.Avoid repeated freeze-thaw cycles.

·As an alternative to the above, sample stability may be established by each laboratory.





New Coronavirus COVID-19 Neutralizing Antibody ELISA Kit Operating Instruction



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and Health Industrial Park, No.1048 Zhong Qing Road, Kai Fu 
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