 |
25 tests/kit & 50 tests/kit
Hunan Runmei Gene
RM-D-A1001
L/C, T/T, D/P, Western Union, Paypal
CE, ISO 13485
Nucleic Acid Isolation Kit / Viral RNA Extraction Kit / Virus DNA Extraction Kit
Virus Nucleic Acid Extraction Kit or Nucleic Acid Detection Kit Description
The Virus Nucleic Acid Extraction Kit or Nucleic Acid Detection Kit uses a unique buffer system and a centrifugal adsorption column that specifically binds virus DNA/RNA. Centrifugal adsorption columns can efficiently and specifically adsorb viral DNA/RNA, and remove impurities and proteins as much as possible. It is widely used to extract virus DNA/RNA from plasma, serum, ascites, cell culture supernatant, cerebrospinal fluid, stool, tissue and other samples. The extracted nucleic acid can be used for clinical molecular biology detection.
Virus Nucleic Acid Extraction Kit or Nucleic Acid Detection Kit Features
1. Simple operation:
It takes a short time and can be learned by simple training, which can quickly extract various samples.
2. Short extraction time:
Depending on the sample flux, 16 to 32 fluxes can be extracted in 25 minutes.
3. No need to heat and boil:
Chemical cracking at room temperature, without heating and boiling.
4. High nucleic acid concentration and high sensitivity:
The extracted nucleic acid has less loss and the detection sensitivity is higher.
5. High purity:
The nucleic acid absorbance ratio OD260/OD280 is between 1.8 and 2.2.
Specifications and Models
| Name | Number | Packing unit |
Virus Nucleic Acid Extraction Kit or Nucleic Acid Detection Kit | 50 | Box |
Weight Volume
No alcohol
Unit | Piece | Volume(CM) | Weight(KG) |
Per box | 50 | 15.6*11*11 | 0.25 |
per carton | 52*50 | 65*40*45 | 14.6 |
| per pallet | 14*52*50 | 120*105*135 | 204.4 |
No alcohol, no dry ice and no ice packs
Unit | Piece | Volume(CM) | Weight(KG) |
Per box | 50 | 15.6*11*11 | 0.275 |
per carton | 52*50 | 65*40*45 | 15.9 |
| per pallet | 14*52*50 | 120*105*135 | 222.6 |
Intended Use
This kit is used to extract virus DNA/RNA from plasma, serum, ascites, cell culture supernatant, cerebrospinal fluid, stool, tissue and other samples. The extracted nucleic acid can be used for clinical molecular biology detection.
Product Principle
This kit uses a unique buffer system and a centrifugal adsorption column that specifically binds virus DNA/RNA. Centrifugal adsorption columns can efficiently and specifically adsorb viral DNA/RNA, abd remove impurities and proteins as much as posssible.
Main Ingredients
| Name | Composition | 50 Testing/Box |
| 1 | Adsorption Column | 50x1 Pack |
| 2 | Collection Tube | 50x1 Pack |
| 3 | Lysis Solution | 11ml x 1 Bottle |
| 4 | Wash Buffer I | 14ml x 1 Bottle (and 18ml absolite ethanol before the first use) |
| 5 | Wash Buffer II | 8ml x 1 Bottle (and 24ml absolite ethanol before the first use) |
| 6 | Elution Solution | 3ml x 1Bottle |
| 7 | Manual | 1 serving |
Storage Conditions and Validity
Store at normal temperature 5-25 ℃, valid for 12 months.
Self Provided Materials
Sterilized 1.5ml centrifuge tube, centrifuge, absolute ethanol, vortex mixer, etc.
Sample Requirements
1. Samples can be extracted immediately after ollection. If they cannot be extracted immediately, they can be stored refrigerated at 2-8 ℃ overnight. For long-term storage, specimens should be frozen at -20 ℃ to -80 ℃.
2. Avoid repeated freeze-thaw cycles during sample storage.
Sample Preparation
1. Animal and plant tissues: The samples are sufficiently ground with physiological saline or PBS buffer, and the supernatant is centrifuged to perform the sample extraction operation.
2. Serum, plasma, body fluid and other samples: The samples extraction can be performed directly (less than 200μl, PBS buffer or physiol ogical saline can be added to make up 200μl).
Instructions
1. Take 200ul of plasma, serum, ascites, cell culture supernatant, cerebrospinal fluid, tissue milled supernatant and other liquids (do not use whole blood), place in a clean 1.5ml centrifuge tube, add 200μl Lysis Solution to the centrifuge tube, vortex and shake for 10s.
2. Let stand for 10 minutes at room temperature, then add 200ul of absolute ethanol to the centrifuge tube, vortex and shake for 10s.
3. Put the Adsorption Column into the Collection Tube, transfer all the above liquid into the Adsorption Column, centrifuge at 8000rpm for 1 minute, and discard the filtrate.
4. Add 600ul of Washing Buffer I to the Adsorption Column (confirm that absolute ethanol was added to Washing Solution I before use), and centrifuge at 8000rpm for 1 minute, and discard the filtrate.
5. Add 600ul of Washing Buffer II to the Adsorption Column(confirm that absolute ethanol was added to Washing Solution II before use), centrifuge at 8000rpm for 1
minute, and discard the filtrate.
6. Return the Adsorption Column to the collection tube and centrifuge at 8000rpm for 2 minutes, discard the filtrate and the collection tube.
7. Place the Adsorption Column in a RNase/DNase-free 1.5ml centrifuge tube, add 50μl Elution Solution in the center of the Adsorption Column membrane, and leave it at room temperature for 1 minute. Centrifuge at 8000rpm for 1 minute, discard the Adsorption Column. The nucleic acid are collected in the 1.5 ml centrifuge tube was stored at -20 ℃.
Virus Nucleic Acid Extraction Kit or Nucleic Acid Detection Kit Description
The Virus Nucleic Acid Extraction Kit or Nucleic Acid Detection Kit uses a unique buffer system and a centrifugal adsorption column that specifically binds virus DNA/RNA. Centrifugal adsorption columns can efficiently and specifically adsorb viral DNA/RNA, and remove impurities and proteins as much as possible. It is widely used to extract virus DNA/RNA from plasma, serum, ascites, cell culture supernatant, cerebrospinal fluid, stool, tissue and other samples. The extracted nucleic acid can be used for clinical molecular biology detection.
Virus Nucleic Acid Extraction Kit or Nucleic Acid Detection Kit Features
1. Simple operation:
It takes a short time and can be learned by simple training, which can quickly extract various samples.
2. Short extraction time:
Depending on the sample flux, 16 to 32 fluxes can be extracted in 25 minutes.
3. No need to heat and boil:
Chemical cracking at room temperature, without heating and boiling.
4. High nucleic acid concentration and high sensitivity:
The extracted nucleic acid has less loss and the detection sensitivity is higher.
5. High purity:
The nucleic acid absorbance ratio OD260/OD280 is between 1.8 and 2.2.
Specifications and Models
| Name | Number | Packing unit |
Virus Nucleic Acid Extraction Kit or Nucleic Acid Detection Kit | 50 | Box |
Weight Volume
No alcohol
Unit | Piece | Volume(CM) | Weight(KG) |
Per box | 50 | 15.6*11*11 | 0.25 |
per carton | 52*50 | 65*40*45 | 14.6 |
| per pallet | 14*52*50 | 120*105*135 | 204.4 |
No alcohol, no dry ice and no ice packs
Unit | Piece | Volume(CM) | Weight(KG) |
Per box | 50 | 15.6*11*11 | 0.275 |
per carton | 52*50 | 65*40*45 | 15.9 |
| per pallet | 14*52*50 | 120*105*135 | 222.6 |
Intended Use
This kit is used to extract virus DNA/RNA from plasma, serum, ascites, cell culture supernatant, cerebrospinal fluid, stool, tissue and other samples. The extracted nucleic acid can be used for clinical molecular biology detection.
Product Principle
This kit uses a unique buffer system and a centrifugal adsorption column that specifically binds virus DNA/RNA. Centrifugal adsorption columns can efficiently and specifically adsorb viral DNA/RNA, abd remove impurities and proteins as much as posssible.
Main Ingredients
| Name | Composition | 50 Testing/Box |
| 1 | Adsorption Column | 50x1 Pack |
| 2 | Collection Tube | 50x1 Pack |
| 3 | Lysis Solution | 11ml x 1 Bottle |
| 4 | Wash Buffer I | 14ml x 1 Bottle (and 18ml absolite ethanol before the first use) |
| 5 | Wash Buffer II | 8ml x 1 Bottle (and 24ml absolite ethanol before the first use) |
| 6 | Elution Solution | 3ml x 1Bottle |
| 7 | Manual | 1 serving |
Storage Conditions and Validity
Store at normal temperature 5-25 ℃, valid for 12 months.
Self Provided Materials
Sterilized 1.5ml centrifuge tube, centrifuge, absolute ethanol, vortex mixer, etc.
Sample Requirements
1. Samples can be extracted immediately after ollection. If they cannot be extracted immediately, they can be stored refrigerated at 2-8 ℃ overnight. For long-term storage, specimens should be frozen at -20 ℃ to -80 ℃.
2. Avoid repeated freeze-thaw cycles during sample storage.
Sample Preparation
1. Animal and plant tissues: The samples are sufficiently ground with physiological saline or PBS buffer, and the supernatant is centrifuged to perform the sample extraction operation.
2. Serum, plasma, body fluid and other samples: The samples extraction can be performed directly (less than 200μl, PBS buffer or physiol ogical saline can be added to make up 200μl).
Instructions
1. Take 200ul of plasma, serum, ascites, cell culture supernatant, cerebrospinal fluid, tissue milled supernatant and other liquids (do not use whole blood), place in a clean 1.5ml centrifuge tube, add 200μl Lysis Solution to the centrifuge tube, vortex and shake for 10s.
2. Let stand for 10 minutes at room temperature, then add 200ul of absolute ethanol to the centrifuge tube, vortex and shake for 10s.
3. Put the Adsorption Column into the Collection Tube, transfer all the above liquid into the Adsorption Column, centrifuge at 8000rpm for 1 minute, and discard the filtrate.
4. Add 600ul of Washing Buffer I to the Adsorption Column (confirm that absolute ethanol was added to Washing Solution I before use), and centrifuge at 8000rpm for 1 minute, and discard the filtrate.
5. Add 600ul of Washing Buffer II to the Adsorption Column(confirm that absolute ethanol was added to Washing Solution II before use), centrifuge at 8000rpm for 1
minute, and discard the filtrate.
6. Return the Adsorption Column to the collection tube and centrifuge at 8000rpm for 2 minutes, discard the filtrate and the collection tube.
7. Place the Adsorption Column in a RNase/DNase-free 1.5ml centrifuge tube, add 50μl Elution Solution in the center of the Adsorption Column membrane, and leave it at room temperature for 1 minute. Centrifuge at 8000rpm for 1 minute, discard the Adsorption Column. The nucleic acid are collected in the 1.5 ml centrifuge tube was stored at -20 ℃.